Electron capture dissociation of peptides metalated with alkaline-earth metal ions
نویسندگان
چکیده
منابع مشابه
Electron capture dissociation of oligosaccharides ionized with alkali, alkaline Earth, and transition metals.
We extend the application of electron capture dissociation (ECD) (which requires at least two charges) to oligosaccharides without basic functionalities by utilizing alkali, alkaline earth, and transition metals (Na+, K+, Ca2+, Ba2+, Mg2+, Mn2+, Co2+, and Zn2+) as charge carriers in electrospray ionization. Both linear and branched oligosaccharides were examined, including maltoheptoase, p-lact...
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We compare electron capture dissociation (ECD) of doubly protonated and divalent metal-adducted tyrosine O-sulfated peptides without basic amino acid residues. ECD of doubly protonated Tyr2-sulfated cholecystokinin (CCKS) and doubly protonated Tyr12-sulfated gastrin II (GST) resulted in complete loss of SO3 from all product ions. Thus, contrary to typical ECD behavior, localization of the sulfa...
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We have investigated electron capture dissociation (ECD) of doubly protonated peptides with few or no basic amino acid residues (BAARs). For peptides containing one His, abundant b-type ions were only found when His was located adjacent to the N-terminus. Interestingly, b-type ions, particularly b(5)(+), were found to be the dominant product ions in ECD of peptides without BAARs. Fragmentation ...
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Analysis of phosphotyrosine and phosphoserine containing peptides by nano-electrospray Fourier transform ion cyclotron resonance (FTICR) mass spectrometry established electron capture dissociation (ECD) as a viable method for phosphopeptide sequencing. In general, ECD spectra of synthetic and native phosphopeptides appeared less complex than conventional collision activated dissociation (CAD) m...
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ژورنال
عنوان ژورنال: Journal of the American Society for Mass Spectrometry
سال: 2006
ISSN: 1044-0305,1879-1123
DOI: 10.1016/j.jasms.2006.01.014